 |
 |
 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Chemioresistance of an Adriamycin-Selective Human Small-Cell Lung Carcinoma Cell Line) ผู้เขียน: ดร.สมจิตต์ ปาละกาศ, อาจารย์ , นางพรรณี พักคง, รองศาสตราจารย์ ,  Krongchan Ratanaphadit สื่อสิ่งพิมพ์:pdf AbstractThe resistance to cytotoxic anticancer drugs of two human small-cell lung cancer cell lines was determined for the parental GLC4 cell line and the adriamycin-selective GLC4/Adr cell line. In comparison with their parental counterparts, the GLC4/Adr cells displayed higher resistance to all test compounds, including doxorubicin, vincristine and vinblastine, with respective IC50 values of 3.553 ? 0.247, 9.630 ? 0.134 and 0.858 ? 0.072 nM leading to estimates of resistance factors of 310 ? 29, 32 ? 5 and 2.7 ? 0.3, respectively. These findings indicated that drug-resistant cells generated by in vitro selection in the presence of step-wise concentration of a single cytotoxic drug could display some of the characteristics associated with the overexpression of the membrane transporter protein responsible for cellular multispecific detoxification. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Cellular Chemo-Resistance and Radiosensitivity of Parental and Adriamycin-Selective Human Small-Cell Lung Cancer Cell Lines) ผู้เขียน:Yanitsa Chantapoon, Krongchan Ratanaphadit, ดร.สมจิตต์ ปาละกาศ, อาจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe radiosensitivity of two small-cell, lung cancer cell lines comprising the adriamycin-selective cell line (GLC4/Adr) and its parental counter cell line (GLC4) was investigated in an aqueous soluble system. A simplified resazurin assay was optimized and used in the cell viability investigation. Cell survival was evaluated after being exposed to cytotoxic agents, deduced from the resazurin reduction using the optical density of a spectrophotometer instead of a fluorescent signal. Compared with their parental counterparts, the GLC4/Adr cells displayed a high resistance capability to doxorubicin with a resistant factor of about 320. No significant resistance to gamma radiation was demonstrated after exposure to gamma radiation from a Cs-137 source. The GLC4/Adr cell line responded to radiation in the same manner as its parental counterpart. This finding suggested that a drug resistance phenotype occurred in the GLC4/Adr cells (plausibly acquired after being cultured in the presence of oxidative stress produced by the step-wise concentration of adriamycin) and it is not responsible for the radioresistance of the cells. |
 |
|
|